用基因枪将人工构建的植物雄性不育基因ＴＡ２９－Ｂａｒｎａｓｅ及其标记基因ｂａｒ导入芝麻豫芝４号的离体子叶，在ＭＳ＋５ｍｇ·Ｌ－１６－ＢＡ＋１ｍｇ·Ｌ－１ＩＡＡ＋１ｍｇ·Ｌ－１ＡＢＡ＋５ｍｇ·Ｌ－１ＡｇＮＯ３的培养基上交培养１２天，转入光、暗光替培养，诱导出不定芽。经添加２ｍｇ·Ｌ－１除草剂（ＢＡＳＴＡ）的上述培养基筛选，获得了１１个ＢＡＳＴＡ抗性株系，对其中３个株系进行Ｓｏｕｔｈｅｒｎ杂交鉴定分析，证实其中２个为转基因株系，转化率为１．４６％ The constructed plant male sterility gene TA29-Barnase and its marker gene bar were introduced into the excised cotyledons of sesame Yuzhi 4 with a gene gun, and then cultured in MS + 5 mg · L-16-BA + 1 mg · L-1 IAA + 1 mg · L -1 ABA + 5 mg · L- 1AgNO3 culture medium for 12 days, transferred to light, dark light for the culture, inducing adventitious buds. Eleven BASTA resistant strains were screened by the above medium supplemented with 2 mg · L-1 herbicide (BASTA). Three of the three strains were identified by Southern hybridization and confirmed that two of them were transgenic lines, Rate was 1.46%