我们于1996年开始进行经胰腺供血动脉置管灌注5-FU、奥曲肽治疗急性坏死性胰腺炎的动物实验研究,取得成功后,于1999年应用于临床,取得良好疗效,并对其作用机制、胰酶抑制剂组合应用方案、临床适应症以及疗效评价方法进行系统化研究,现归纳如下:1.动物实验研究将健康杂种犬随机分成四组:对照组、5-FU组、奥曲肽组和5-FU联合奥曲肽组,各组参照Aho法经主胰管逆行注射5%牛磺胆酸钠,量按1ml/k计算,制备成急性出血坏死性胰腺炎模型。之后,经胃网膜右动脉插管至胃十二指肠 In 1996, we started the experimental study on the perfusion of 5-FU via cannulation of pancreatic feeding artery and octreotide treatment of acute necrotizing pancreatitis. After successful treatment, we applied it in clinical practice in 1999 and obtained good curative effect. The combination of pancreatic enzyme inhibitor application programs, clinical indications and efficacy evaluation methods for systematic study, are summarized as follows: 1. Animal Experimental Study Healthy mongrel dogs were randomly divided into four groups: control group, 5-FU group, octreotide group and 5 -FU combined octreotide group, each group according to the Aho method through the main pancreatic duct retrograde injection of 5% sodium taurocholate, the amount of 1ml / k calculated to prepare acute hemorrhagic necrotizing pancreatitis model. After that, through the right gastric artery intubation to the gastroduodenal