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Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%～95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
Based on this, a novel method for the determination of sparfloxacin in human urine. The linear The recoveries are 91.5% -95.7% and the RSD is 1.2% -4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.