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目的:通过测定口腔溃疡黏膜上皮细胞,研究细胞核内DNA分布区的改变。方法:在丁卡因表麻下用小毛刷刷取口腔溃疡处上皮细胞,经固定后,制成1~2张玻片,行Feulgen染色做DNA倍体测定,将出现DNA分布区变化病例的溃疡组织活检行病理组织检查。结果:46例病例中有12例在口腔异倍体细胞定量测定后发现5c(7%±2.6%)、7c(11%±4.3%)和9c(16%±5%)细胞核内高DNA分布区明显较2c细胞(2%±1.2%)增多(t-配对比较,P﹤0.05)。12例病例病理证实7例中度异常增生、4例异常增生、1例浸润癌。结论:口腔溃疡病变组织中、重度不典型增生和浸润癌的病例可出现DNA倍体异常并伴有核内高DNA分布区增大。
Objective: To study the changes of DNA distribution in the nucleus by measuring oral ulcer mucosal epithelial cells. Methods: The epithelial cells of oral ulcer were swabbed with small brush under the tetracaine table, and after fixation, 1 to 2 slides were made. DNA digestion was performed by Feulgen staining. Ulcer tissue biopsy line histopathology. RESULTS: Twelve out of 46 cases were found to have high DNA distribution in the nucleus of 5c (7% ± 2.6%), 7c (11% ± 4.3%) and 9c (16% ± 5%) after quantitative measurement of oral aneuploid cells Compared with 2c cells (2% ± 1.2%), the area increased significantly (t-paired comparison, P <0.05). Pathological examination of 12 cases confirmed 7 cases of moderate dysplasia, 4 cases of abnormal proliferation and 1 case of invasive carcinoma. CONCLUSION: DNA ploidy abnormalities may be found in cases of severe oral dysplasia and severe dysplasia and invasive carcinoma accompanied with an increase in the distribution of high DNA in the nucleus.