论文部分内容阅读
目的从生物物理和生物化学的角度,探讨黄连与吴茱萸分煎后配伍时的药性差异。方法黄连和吴茱萸药材用水分别煎煮,分煎液按相应的比例配伍,在100℃下水浴加热1 h,得单味药分煎后配伍所得的左金丸、甘露散、茱萸丸和反左金丸药液。采用微量热法,测定了痢疾杆菌在类方水提液作用下的生长代谢热谱曲线,获得相应的生物热动力学参数,结合文献报道,综合分析黄连与吴茱萸分煎后配伍时的药性差异。结果黄连与吴茱萸分煎后配伍的药液之间存在稳定的药性差异。左金丸及类方均能不同程度地抑制痢疾杆菌的生长代谢过程,左金丸的抑制作用最强,反左金丸的抑制作用最弱,4方抑菌作用强弱顺序为:左金丸>甘露散>茱萸丸>反左金丸。结论微量热法可用于刻画黄连与吴茱萸分煎后配伍时的差异。
OBJECTIVE: To investigate the differences of pharmacodynamics between Coptis chinensis and Fructus Evodiae decocted after decocted by biophysical and biochemical methods. Methods Coptis and Evodia decoction of herbs were decocted, the decoction of the corresponding proportion of compatibility, heated at 100 ℃ water bath 1 h, were single herb after decoction of compatibility Zuojin pills, mannose powder, dogwood pills and anti-Zuo Jin pill liquid. The microcalorimetric method was used to determine the growth metabolic heat spectrum curve of dysentery bacillus in the aqueous extract of Rhizoma et Radix and to obtain the corresponding thermokinetic parameters. Combined with the literature reports, . Results Coptis and Evodia decoction after the compatibility of the liquid medicine there is a stable difference between the drug. Zuo Jin Wan and other similar prescriptions can inhibit the growth and metabolism of Shigella dysenteriae to a certain extent. Zuojin Wan has the strongest inhibitory effect and the anti-Zuojin Wan has the weakest inhibitory effect. The order of the four antibacterial activities is Zuojinwan> Ganluosan > Cornel pill> Anti-Zuo Jin Wan. Conclusion Calorimetry can be used to characterize the difference between the compatibility of Coptis chinensis and Fructus Evodiae decocted.