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目的:研究内皮细胞特异分子-1(endothelial cell specific-1,endocan)、血管内皮细胞生长因子受体(KDR/FLK-1)在非小细胞肺癌(NSCLC)中的表达及其临床意义,同时探讨endocan、FLK-1与非小细胞肺癌血管生成的相关性。方法:应用免疫荧光技术检测60例手术切除的肺癌组织、21例癌旁肺组织(距癌组织5cm以上)中的endocan表达水平;免疫组化SP法检测FLK-1表达,并计数微血管密度(MVD)。结果:endocan、FLK-1的表达在NSCLC组织中明显高于癌旁组织(P均<0.05);endocan、FLK-1的表达与NSCLC的淋巴结转移、TNM分期正相关(P均<0.05),而与NSCLC的组织学分类无关(P均>0.05);在NSCLC中MVD、endocan、FLK-1阳性表达组明显高于二者阴性表达组(P均<0.05);endocan、FLK-1在NSCLC中的表达呈正相关(r=0.8889,P<0.05)。结论:endocan、FLK-1在NSCLC组织的血管发生、进展等病理过程有重要作用。
Objective: To investigate the expression and clinical significance of endothelial cell-specific end-endocan and vascular endothelial growth factor receptor (KDR / FLK-1) in non-small cell lung cancer (NSCLC) To investigate the correlation between endocan, FLK-1 and angiogenesis in non-small cell lung cancer. Methods: Immunofluorescence was used to detect endocan expression in 60 surgically resected lung cancer tissues and 21 paracancer lung tissues (more than 5 cm away from the cancer). The expression of FLK-1 was detected by immunohistochemical SP method and the microvessel density MVD). Results: The expression of FLK-1 and endocan in NSCLC tissues was significantly higher than that in adjacent non-cancerous tissues (P <0.05). The expression of endocan and FLK-1 was positively correlated with lymph node metastasis and TNM stage in NSCLC (all P <0.05) (P <0.05). The positive rates of MVD, endocan and FLK-1 in NSCLC were significantly higher than those in NSCLC (P <0.05). Endocan and FLK-1 in NSCLC (R = 0.8889, P <0.05). Conclusion: Endocan and FLK-1 play an important role in the pathogenesis of NSCLC such as angiogenesis and progression.