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Objective:To investigate the expression of HIP/PAP in hepatocellular carcinoma(HCC)patients and explore its role in migration and invasion of HCC.Methods:The expression of HIP/PAP in HCC tissue and corresponding adjacent noncancerous tissue was assessed by IHC,RT-PCR and Western blot.The correlation between clinicopathological features and HIP/PAP expression was analyzed.The role of HIP/PAP on invasion and migration of HCC cells was observed by RNA interference,wound healing and Transwell assay.Results:Both mRNA and protein expression of HIP/PAP was upregulated in HCC tissues compared to tumor-adjacent tissue and correlated with poor tumor differentiation,advanced tumor stage and vascular invasion.HIP/PAP expression was also upregulated in HCC cells,and silencing its expression by specific siRNA could inhibit the invasion and migration of HCC cells.Conclusions:HIP/APA is overexpressed in HCC and contributes to the migration and invasion of HCC cells.
Objective: To investigate the expression of HIP / PAP in hepatocellular carcinoma (HCC) patients and explore its role in migration and invasion of HCC. Methods: The expression of HIP / PAP in HCC tissue and corresponding adjacent noncancerous tissue was assessed by IHC, RT -PCR and Western blot.The correlation between clinicopathological features and HIP / PAP expression was analyzed. The role of HIP / PAP on invasion and migration of HCC cells was observed by RNA interference, wound healing and Transwell assay. Results: Both mRNA and protein expression of HIP / PAP was upregulated in HCC tissues compared to tumor-adjacent tissue and correlated with poor tumor differentiation, advanced tumor stage and vascular invasion. HIP / PAP expression was also upregulated in HCC cells, and silencing its expression by specific siRNA could inhibit the invasion and migration of HCC cells. Conclusions: HIP / APA is overexpressed in HCC and contributes to the migration and invasion of HCC cells.