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探讨表面结合聚（２－乙基丙烯酸）后脂质小囊的体外ｐＨ敏感性。方法将巯基化的聚（２－乙基丙烯酸）连接在含有二肉豆蔻酰－Ｎ－［（４－马来酰亚胺甲基）环己羰基］磷脂酰乙醇胺的类脂双分子层表面，制备ｐＨ－敏感型脂质小囊。以内容物钙黄绿素酸化前后体外释放百分率的变化作为指标，考察不同相对分子质量的聚（２－乙基丙烯酸）和不同离子强度磷酸盐缓冲液对脂质小囊ｐＨ敏感性的影响。结果高相对分子质量聚（２－乙基丙烯酸）表面结合脂质小囊对ｐＨ最敏感；以１００ｍｍｏｌ／Ｌ磷酸盐缓冲液为介质制备的脂质小囊ｐＨ敏感性最好。结论聚（２－乙基丙烯酸）表面结合的脂质小囊具有ｐＨ敏感性
To investigate the in vitro pH sensitivity of surface-bound poly (2-ethylacrylate) lipid vesicles. Methods The thiolated poly (2-ethyl acrylic acid) was attached to the surface of the lipid bilayer containing dimyristoyl-N - [(4-maleimidomethyl) cyclohexanecarbonyl] phosphatidylethanolamine. PH-sensitive lipid vesicles were prepared. The change of in vitro release percentage before and after calcein acidification was used as an index to investigate the effect of poly (2-ethylacrylate) and different ionic strength phosphate buffer solutions on the pH sensitivity of lipid vesicles. Results The lipid vesicles with high relative molecular weight poly (2-ethylacrylate) surface were the most sensitive to pH. The lipid vesicles prepared with 100 mmol / L phosphate buffer had the best pH sensitivity. Conclusions Poly (2-ethylacrylate) surface-bound lipid vesicles are pH-sensitive