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目的探讨宫颈癌FHIT(脆性组胺酸三联体基因)、PTEN(10号染色体缺失磷酸酶及张力蛋白同源物基因)甲基化与hMSH2表达在宫颈癌发病中的意义;方法通过对宫颈癌以及CIN病理标本,用甲基化特异性PCR检测其FHIT、PTEN甲基化、用免疫组化学法检测错配修复基因(hMSH2)表达;结果宫颈癌、CIN及慢性宫颈炎组,FHIT甲基化阳性表达率分别为56.7(17/30)、14.3(5/35)、0(0/43)(P<0 05);PTEN蛋白阳性表达率分别为23.3(7/30)、5.7(2/35)、0(0/43)(P<0 05);hMSH2蛋白阳性表达率分别为73.3(22/30)、42.8(15/35)(4/19)、20.9(9/43)(P<0 05)。结论宫颈癌的发病过程中伴有FHIT、PTEN甲基化、hMSH2蛋白表达上调,但三者机制各自独立。三者联合检测有助于宫颈癌的预警和愈后诊断。
Objective To investigate the significance of the methylation status of hFHIT (FHIT) and PTEN (chromosome 10 deletion phosphatase and tensin homologue) and the expression of hMSH2 in cervical cancer. And CIN pathological specimens. The methylation of FHIT and PTEN was detected by methylation-specific PCR and the expression of mismatch repair gene (hMSH2) was detected by immunohistochemistry. Results Cervical cancer, CIN and chronic cervicitis group, FHIT methyl The positive expression rate of PTEN protein was 56.7 (17/30), 14.3 (5/35) and 0 (0/43) respectively (P <0.05) / 35), 0 (0/43), respectively (P <0.05). The positive rates of hMSH2 protein were 73.3 (22/30), 42.8 (15/35) (4/19), 20.9 P <0 05). Conclusion The pathogenesis of cervical cancer accompanied by FHIT, PTEN methylation, hMSH2 protein expression increased, but the three mechanisms are independent. Joint detection of the three cervical cancer help early warning and prognosis.