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本文作者为探索β-干扰素在抗弓形虫感染及弓形虫病治疗上的作用,分别采用体外实验和体内实验的方法来研究。在实验中,选用Swiss Webster,BALB/C,CBA/Ca小鼠作为实验动物,分别从其腹腔液中收集弓形虫RH株、C56株的速殖子和ME49株的包囊、收集腹腔巨噬细胞。然后将小鼠腹腔巨噬细胞与各种浓度的重组鼠β-干扰素(rMuIFN-β)孵育过夜,再感染弓形虫RH株。通过检测感染细胞的百分比
In order to explore the role of interferon-beta in the treatment of Toxoplasma gondii infection and toxoplasmosis, the present study was conducted in vitro and in vivo respectively. In the experiment, Swiss Webster, BALB / C and CBA / Ca mice were selected as experimental animals, Toxoplasma gondii RH strain, tachyzoites of C56 strain and ME49 strain were collected from the peritoneal fluid, and the peritoneal macrophages cell. Mice peritoneal macrophages were then incubated with various concentrations of recombinant murine beta-interferon (rMuIFN-beta) overnight prior to infection with T. gondii RH strain. By detecting the percentage of infected cells