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用云南山楂(Crataegus scabrifolia(Franch.)Rehd.)成年树茎尖和实生芽两种不同发育阶段的材料为外殖体,诱导它们休眠芽萌动,丛生芽条并诱导芽条生根。实验结果如下:1.以成年态的云南山楂侧芽为外植体,培养在附加IAA 0.1—0.5mg/l+6-BA 1-2mg/l的MS培养基上可诱导芽的萌发;将芽继代培养在附加0.5—1mg/l 6-BA的SH或MS培养基上,40天后芽数增殖4—6倍;将芽条截下置于1/2MS培养基上,附加不同浓度的IAA或IBA,可得到50—80％的生根率。2.以实生芽为外殖体,在相同条件下,则20天后芽数增殖便可获4—6倍;98％以上生根。结果表明:云南山楂的幼年态要比成年态易脱分化和再分化。
The materials of two different developmental stages of shoot tip and shoot of adult Crataegus scabrifolia (Franch.) Rehd. Were used as explants to induce germination of dormant buds, clustered shoots and root induction of shoots. The experimental results are as follows: 1. In the adult state of Yunnan hawthorn lateral bud explants, the buds can be induced to germinate on MS medium supplemented with IAA 0.1-0.5mg / l and 6-BA 1-2mg / l; The subcultures were propagated 4-6 times on the SH or MS medium with 0.5-1 mg / l 6-BA added after 40 days. The shoots were cut and placed on 1/2 MS medium supplemented with different concentrations of IAA Or IBA, a rooting rate of 50-80% is obtained. 2. With real buds as exosomes, under the same conditions, then 20 days after the bud proliferation can be 4-6 times; 98% above rooting. The results showed that the juvenile state of hawthorn in Yunnan was easier to dedifferentiate and re-differentiate than the adult state.