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胰岛素对外周细胞的作用可以借受体水平上或受体后水平上的改变进行调节。以往曾报导过,在一些抗胰岛素的临床病例有受体结合降低和受体后缺陷。这些都是胰岛素效应调节的长期变化而对于胰岛素作用于人类外周组织的急性适应了解甚少。现在已建立了应用~(125)I-(Tyr-A14)-胰岛素作结合测定和生物萤光技术测定脂解作用的方法,这些方法可同时分析人类脂肪微量样品中胰岛素结合和胰岛素的抗脂解效应。本文报告了作者应用这些方法研究胰岛素在人类脂肪细胞中作用的急性影响。受试者为8名体重正常的成人(4名男人;4
The effect of insulin on peripheral cells can be regulated by changes in the level of the receptor or at the level of the receptor. It has been previously reported that in some insulin-resistant clinical cases there is a decrease in receptor binding and post-receptor defects. These are long-term changes in the regulation of insulin effects and little is known about the acute adaptation of insulin to human peripheral tissues. A method for the determination of lipolysis using ~ (125) I- (Tyr-A14) -insulin binding assay and bioluminescence assay has now been established. These methods can simultaneously analyze insulin binding and insulin resistance in human fat micro-samples Solution effect. This article reports the authors’ use of these methods to study the acute effects of insulin on human adipocytes. Subjects were 8 normal-weight adults (4 men; 4