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目的:应用离体培养乳鼠胰岛细胞,探讨1,6-二磷酸果糖(fructose-1,6-diphosphate,FDP)对白细胞介素1β(IL-1β)损伤的胰岛细胞是否具有保护作用。方法:实验选用出生1～3d的Wistar大鼠20只。分离培养后的胰岛细胞,将其随机分为对照组、FDP组、IL-1β损伤组、IL-1β+FDP组,每组平行孔为6孔。应用MTT法检测对照组、IL-1β损伤组以及不同浓度(2.5,5.0,7.5,10.0,12.5,15.0mmol/L)FDP保护组胰岛细胞的细胞活性。结果:IL-1β损伤组细胞活性(A值:0.116±0.012)明显低于对照组(0.252±0.020)(F=8.92,P<0.01),加入5～10mmol/LFDP可使细胞活性增强(F=13.35,22.56,P<0.01),但2.5,12.5,15.0mmol/LFDP与受损细胞共同孵育后未能使细胞活性升高(P>0.05)。10,40h时未见FDP对受损细胞活性的保护,20h时IL-1β+FDP组细胞活性(A值:0.219±0.004)明显高于IL-1β损伤组(0.178±0.010)(F=19.10,P<0.01),30hIL-1β+FDP组细胞活性明显高于IL-1β损伤组(F=16.05,P<0.01)。结论:FDP对IL-1β损伤的胰岛细胞活性具有保护作用,且具有时间和剂量依从关系。
OBJECTIVE: To investigate whether fructose-1,6-diphosphate (FDP) can protect islet cells damaged by interleukin-1β (IL-1β) in cultured rat islet cells. Methods: Twenty (20) Wistar rats of 1 to 3 days of age were selected for the experiment. The cultured islet cells were isolated and randomly divided into control group, FDP group, IL-1β injury group and IL-1β + FDP group. Each group of parallel wells was 6 wells. The cell viability of islet cells in control group, IL-1β injury group and FDP protection group at different concentrations (2.5, 5.0, 7.5, 10.0, 12.5, 15.0 mmol / L) RESULTS: The cell viability (A value: 0.116 ± 0.012) in IL-1β group was significantly lower than that in control group (0.252 ± 0.020, F = 8.92, P <0.01) = 13.35,22.56, P <0.01). However, incubation with 2.5,12.5,15.0 mmol / L LFDP failed to increase cell viability (P> 0.05). The protective effect of FDP on injured cells was not observed at 10 and 40h, and the cell viability (A value: 0.219 ± 0.004) in IL-1β + FDP group was significantly higher than that in IL-1β group (0.178 ± 0.010) at 20h , P <0.01). The cell viability in 30hIL-1β + FDP group was significantly higher than that in IL-1β group (F = 16.05, P <0.01). Conclusion: FDP has a protective effect on islet cell activity induced by IL-1β, and has time and dose-dependent relationship.