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为确定河南地区杨树黄叶病的病因并提出有效的防治措施,开展了元素补充、组织粉碎回接、微生物分离回接、线虫检测、电子显微镜切片观察、植原体的PCR及DAPI检测等实验。结果表明:对轻度病株施硫酸亚铁有较好的治疗效果;回接组织粉碎物的健株无症状表现;从病株的根、茎、叶中分离了4种微生物并进行了回接,27.5%的接种苗出现了与黄叶病相似的症状;在病株体内没有发现植原体和内寄生性线虫;电子显微镜下观测到了杆状细菌存在。研究结果对该病害的后续研究具有重要的参考价值。
In order to determine the etiological factor of poplar yellow leaf disease in Henan Province and to put forward effective control measures, elemental supplementation, tissue shredding and reconnection, microbial separation and rewinding, nematode detection, electron microscopy, PCR and DAPI detection of phytoplasma were carried out . The results showed that: the treatment of mild diseased ferrous sulfate has a good therapeutic effect; back to the organization ashes the asymptomatic symptom-free performance of the plant; root, stem, leaf from the diseased plants were isolated and carried out four kinds of microbes Then, 27.5% of the inoculated seedlings showed the similar symptoms of yellow leaf disease; no phytoplasma and endoparasitic nematodes were found in the diseased plants; the presence of bacilli was observed under electron microscope. The results of the study of the disease has important reference value.