论文部分内容阅读
目的 观察三苯氧胺（Ｔａｍｏｘｉｆｅｎ， ＴＡＭ）及雌激素（Ｅ２）对乳腺癌ＭＣＦ－７细胞株耐药基因ｍｄｒ－１ｍＲＮＡ水平的影响；方法 应用ＲＴ－ＰＣＲ法观察ＴＡＭ及Ｅ２在不同时间、浓度作用下细胞内ｍｄｒ－１ｍＲＮＡ水平的变化；结果ＴＡＭ可以使ＭＣＦ－７细胞内ｍｄｒ－１ｍＲＮＡ水平降低，而Ｅ２则相反。且以上变化表现出剂量、时间依赖关系。结论ＴＡＭ通过降低ｍｄｒ－１ｍＲＮＡ水平，使肿瘤细胞的耐药性降低，有利于抗肿瘤药物更好的发挥作用。
Objective To observe the effect of tamoxifen (TAM) and estrogen (E2) on the mdr-1 mRNA level of breast cancer MCF-7 cell line; Methods RT-PCR method was used to observe the effects of TAM and E2 on the cell resistance of cells at different time and concentration. Changes in mdr-1 mRNA levels; results TAM can make mdr-1 mRNA levels in MCF-7 cells decreased, while E2 is the opposite. And the above changes show a dose-time dependent relationship. Conclusions TAM can reduce the resistance of tumor cells by decreasing the mdr-1 mRNA level, which is beneficial to the anti-cancer drugs.