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目的研究磁性聚乳酸-羟基乙酸氧化苦参碱纳米粒(M-PLGA-OM-NP)的制备工艺,并对纳米粒子进行评价。方法运用复乳法制备M-PLGA-OM-NP,通过透射电子显微镜观察纳米粒形态,并对纳米粒的平均粒径、载药量、包封率、体外释药情况等进行评价。结果纳米粒外观呈规则球形,其平均粒径为146.5 nm,载药量为7.61%,包封率为44.8%。突释后至第72小时,纳米粒维持较稳定的释药速度,累积释放达52.9%。72～240 h,药物释放缓慢,累计释放约为16.6%,体外释放符合Ritger peppas方程lny=1.280 6+lnt。氧化苦参碱药性不受温度影响。结论获得了较满意的M-PLGA-OM-NP制备工艺,其过程简单,粒子性状符合要求。
Objective To study the preparation of magnetic polylactic-oxyacetic acid oxymatrine nanoparticles (M-PLGA-OM-NP) and to evaluate the nanoparticles. Methods M-PLGA-OM-NP was prepared by double emulsion method. The morphology of the nanoparticles was observed by transmission electron microscopy. The average particle size, drug loading, encapsulation efficiency and in vitro drug release of the nanoparticles were evaluated. Results The nanoparticles showed a regular spherical appearance with an average particle diameter of 146.5 nm, loading capacity of 7.61% and entrapment efficiency of 44.8%. From the time of the burst release to the 72nd hour, the nanoparticles sustained the more stable release rate, with a cumulative release of 52.9%. 72 ~ 240 h, drug release slow, the cumulative release of about 16.6%, in vitro release in line with the Ritger peppas equation lny = 1.280 6 + lnt. Oxymatrine is not affected by temperature. Conclusion The preparation process of M-PLGA-OM-NP is satisfactory. The process is simple and the particle properties meet the requirements.