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目的:建立拆分萘普生对映体的方法。方法:采用超临界流体色谱法。以萘普生对映体的分离时间、容量因子、分离因子、分离度为考察指标,优选手性色谱柱、流动相中极性添加剂种类及占比、背压和柱温的条件。结果:优选条件为手性色谱柱采用CHIRALPAK?AD-H,极性添加剂为异丙醇(占比为20%),背压170 bar,柱温20℃,检测波长283 nm。在此条件下萘普生对映体能够达到基线分离,分离度为4.31,分离因子为1.90,且精密度、稳定性和重复性试验中相关指标的RSD均≤2.65%(n=5)。结论:建立的拆分方法简单、重现性好、拆分效果好,可用于萘普生对映体的手性分离。“,”OBJECTIVE:To develop a method for the separation of naproxen enantiomers. METHODS:The supercritical fluid chromatography(SFC)method was adopted. The chiral column,type and proportion of polar additive in mobile phase,back pres-sure and column temperature were optimized using the separation time,capacity factor,separation factor and separation degree as index. RESULTS:The best condition was on the chiral column CHIRALPAK?AD-H,with polar additive of isopropanol (20%), back pressure of 170 bar,column temperature of 20℃and the detection wavelength of 283 nm. Under this condition,baseline sep-aration of naproxen enantiomers can be achieved;separation degree was 4.31 and separation factor was 1.90;RSD of precision,sta-bility and reproducibility tests were no more than 2.65%(n=5). CONCLUSIONS:Established method is simple,reproducible and well-separated,and can be used for chiral separation of naproxen enantiomers.