成人早老综合征(WS)是一种罕见的常染色体隐性遗传性疾病,以种种的早衰表现为特征.WS患者的皮肤成纤维细胞在体外寿命缩短,生长缓慢和DNA复制速度减慢,但在DNA修复方面无异常.本文研究目的是了解WS细胞自然突变率是否增高.因原代WS细胞增殖能力有限,难于用来分析其自然突变率.故用WS细胞系的SV40转化的细胞株PSV811和W-V,并以SV40转化的正常细胞株W138VA13和AG80B作对照. Premature old age syndrome (WS) is a rare autosomal recessive disease characterized by a variety of premature aging manifestations.While skin fibroblasts in patients with WS have shortened in vitro life, slow growth and slower DNA replication, No abnormality in DNA repair.The purpose of this study is to understand whether the natural mutation rate of WS cells is increased.As a result of the limited ability of primary WS cells to proliferate and difficult to analyze their natural mutation rate, the cell lines transformed with WS40 cell line SV40 PSV811 and WV. The normal cell lines W138VA13 and AG80B transformed with SV40 were used as controls.