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用马铃薯软腐病菌处理烟草后,以化学法测定了叶片中O2-·含量,发现在接种后1￣14h内只出现一次O2-·爆发,不同于以往植物悬浮细胞研究中出现的两次爆发结果。接种软腐病菌后2h和6h烟草叶片O2-·的电子自旋共振波谱图(electronspinresonance,ESR)显示了同样的一次O2-·爆发结果。接种后烟草叶片的线粒体和叶绿体O2-·爆发与叶片的同步,显示此两种细胞器参与烟草叶片在软腐病菌作用下的O2-·发生。无光照叶绿体O2-·的ESR谱线不出现,表明叶绿体O2-·可能来源于光合电子传递链。马铃薯软腐病菌作用下烟草叶片的O2-·爆发是多位点的。
After treatment of tobacco with soft rot of potato, the content of O2- in leaves was measured by chemical method. It was found that there was only one O2- · burst within 1 ~ 14h after inoculation, which was different from the two outbreaks in the past plant suspension cell research result. The electron spin resonance (ESR) of O2- · at 2h and 6h after inoculation with soft rot fungi showed the same result of O2- · burst. After inoculation, the mitochondria and chloroplast O2- · burst of tobacco leaves synchronized with the leaves showed that the two organelles involved in the O2- · generation of tobacco leaves under the action of the soft rot fungi. The ESR spectrum of photon-free chloroplast O2- · does not appear, suggesting that the chloroplast O2- may originate from the photosynthetic electron transport chain. O2- · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · O2 O2 爆 爆 爆 爆 爆 爆 爆 O2