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试验测定了8个粘帚霉Gliocladium spp.菌株的发酵液对3种重要土传病原真菌尖孢镰刀菌Fusarium oxysporum Schlecht.、立枯丝核菌Rhizoctonia solani和核盘菌Sclerotina sclerotiorun的抑菌效果。结果表明,HL-1-1、SH-1-1和SS-1-1的抑菌作用最强,抑菌率分别达83.48％、81.65%和78.68％,3个菌株之间差异不显著;SDT-10-1的抑菌作用最小,抑菌率仅为23.24％。热稳定性测定结果表明:发酵液121℃处理30 min,病原菌生长正常,抑菌作用消失;发酵液经60℃处理后供试菌株的抑菌效果明显,但效果明显降低。
The antibacterial activity of the fermentation broth of eight Gliocladium spp. Strains against Fusarium oxysporum Schlecht, Rhizoctonia solani and Sclerotina sclerotiorun of three important soil-borne pathogens was determined. The results showed that the bacteriostasis of HL-1-1, SH-1-1 and SS-1-1 was the strongest, with the antibacterial rates of 83.48%, 81.65% and 78.68%, respectively. There was no significant difference among the three strains. The antibacterial effect of SDT-10-1 is the smallest, with an antibacterial rate of only 23.24%. The result of thermostability test showed that when the fermentation broth was treated at 121 ℃ for 30 min, the growth of pathogenic bacteria was normal and the antibacterial effect disappeared. The antibacterial effect of the tested strains was obvious at 60 ℃, but the effect was obviously decreased.