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对平邑甜茶叶盘离体再生条件进行了研究 ,结果表明 ,平邑甜茶叶盘愈伤和分化适宜的 BA和 IBA质量浓度分别为 0 .5~ 1 .0和 0 .2 mg/L,愈伤至少需黑暗培养 35d;茎尖分化的适宜培养基为 MS附加 BA 0 .5mg/L、IBA 0 .1~ 0 .2 mg/L ;适宜的生根培养基为1 /2 MS附加 IBA0 .1 mg/L。
The regeneration conditions of P. euphratica leaves were studied in vitro. The results showed that the suitable concentrations of BA and IBA for callus and differentiation of P. orientalis leaves were 0.5 ~ 1.0 and 0.2 mg / L, respectively, The callus at least need dark culture for 35 days. The appropriate culture medium for shoot tip differentiation is MS supplemented with BA 0.5 mg / L and IBA 0 .1-0.2 mg / L, and suitable rooting medium is 1/2 MS supplemented with IBA0. 1 mg / L.