论文部分内容阅读
目的:对小劈碱(BBR)对大肠杆菌的抑制作用的分子靶点进行探讨。方法:鉴于BBR对DNA结合的偏好性,本文从基因转录表达水平对BBR的大肠杆菌生长抑制作用靶点进行实验研究。结果:BBR对于大肠杆菌基因上游调控元件UP element具有较强的亲和力,而在此元件地转录起始区含有TATA碱基序列。进一步对启动子上游含有UP element调控元件的基因sulA、recA、16S和启动子上游不含UP element调控元件的基因lpxC、secG、mutT的mRNA表达比较表明,BBR抑制sulA、recA、16S的表达,对lpxC、secG、mutT无明显作用,提示TATA序列是BBR的作用靶点。结论:本结果对从基因转录水平探讨BBR抑菌作用提供了新思路。
Objective: To investigate the molecular target of the inhibitory effect of mitochondria (BBR) on Escherichia coli. Methods: In view of the preference of BBR for DNA binding, this paper studied the target of growth inhibition of BBR from the level of gene transcription. Results: BBR had a strong affinity to Up element of the upstream regulatory element of E. coli, and the TATA base sequence was contained in the transcription initiation region of this element. Further comparison of the mRNA expression of the genes sulA, recA, 16S containing UP element regulatory elements upstream of the promoter and the genes lpxC, secG and mutT containing no UP regulatory elements upstream of the promoter showed that BBR inhibits the expression of sulA, recA, 16S, The lpxC, secG, mutT no significant effect, suggesting that TATA sequence is the target of BBR. Conclusion: This result provides a new way to explore the bacteriostatic effect of BBR from gene transcription level.