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鲎试剂法(简称LT 法)检查热原具有很多优点,是经典家兔热原检查法(简称RT 法)所无法比拟的。我们用LT 法初选,以RT 法把关,检查各种静脉输注用药近500批次,临床未发现一批结果不符的。我们的经验是:要消除诸多影响LT 法结果准确性的因素,在每做一批药品时都必须严格操作程序,注意下列五个问题.一、抑制试验抑制试验的方法:样品+标准内毒素+鲎试剂。样品用量与样品试验管一致;标准内毒素与阳性对照管用量一致,结果只有在抑制试验与样品之间有明显差异,即前者与阳性试验管一致,而后者
鲎 reagent method (referred to as LT method) Check the pyrogen has many advantages, is the classic rabbit pyrogen test (referred to as RT method) can not be compared. We use the LT method primaries to RT method checks, check nearly 500 batches of intravenous infusion of drugs, the clinical findings did not find a batch of inconsistent. Our experience is: To eliminate a number of factors that affect the accuracy of the LT method, we must strictly follow the procedure at each batch of drugs and pay attention to the following five questions: 1. Method of inhibiting test inhibition test: Sample + standard endotoxin + 鲎 reagent. The amount of the sample is the same as that of the sample test tube; the standard endotoxin is consistent with that of the positive control, and the result is only significant difference between the inhibition test and the sample, that is, the former is consistent with the positive test tube,