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目的 研究牛膝多糖 (ABPS)在体外激活单核细胞的效应。方法 采集健康成人抗凝血 ,用贴壁法分离单核细胞 ,调节细胞数接种到 2 4孔板。在 370 C ,5 %CO2 条件下 ,以同一时间不同ABPS浓度或同一ABPS浓度不同时间培养 ,用流式细胞术和电镜检测单核细胞的激活。结果 ABPS诱导单核细胞超微结构和HLA DR的改变。结论 ABPS上调HLA DR表达并能激活单核细胞。
Objective To study the effect of ABPS on monocyte activation in vitro. Methods Blood was collected from healthy adults. Mononuclear cells were isolated by adherent method and the number of cells was adjusted to 24-well plates. Under the conditions of 370 C, 5% CO2, different ABPS concentrations or different ABPS concentrations at the same time, monocyte activation was detected by flow cytometry and electron microscopy. Results ABPS induced monocyte ultrastructure and HLA DR changes. Conclusion ABPS up-regulates HLA DR expression and activates monocytes.