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目的 应用电镜研究蕲蛇酶对血小板超微结构的影响以探讨用药后血小板减少的原因。 方法 ( 1 )家兔 ( N =6)静脉注射蕲蛇酶前及注射蕲蛇酶 ( 1 AU/kg及 3 AU/kg)后 4 h心脏采血 ,制备血小板标本及超薄切片 ,同时摘取脾及肺按常规制作超薄切片 ,进行电镜观察 ;( 2 )大鼠 ( N =4 )腹腔注射蕲蛇酶 3 AU /kg后分别于 2、8、1 6及 2 4 h摘取脾脏、肺脏制作标本进行电镜观察。 结果 蕲蛇酶 1 AU/kg静脉注射组的家兔 ,血小板表面伸出少数伪足 ,个别凝集或粘连。但内部结构如致密体、α-颗粒、线粒体、开放小管及致密小管系统仍明显可见。 ( 3 AU/kg)剂量组血小板伸出许多突起 ,内部结构大量消失 ,仅见致密小体 ,提示血小板被活化。同时在家兔和大鼠脾脏中见有大量活化变态、内部结构消失的血小板 ,提示血小板活化并被阻隔于脾脏。 结论 蕲蛇酶静脉注射于家兔 ,治疗量对血小板无伤害作用 ,但剂量加大则可使血小板活化 ,脱颗粒终于破坏而被阻隔在脾脏内 ,导致外周血液中血小板计数降低。
Objective To study the effect of Acutobin on the ultrastructure of platelets by electron microscopy to investigate the causes of thrombocytopenia after administration. Methods Rabbits (n = 6) were anesthetized with intravenous injection of viper’s enzyme and 4 h after injection of viper’s enzyme (1 AU / kg and 3 AU / kg) to collect platelets and thin sections, The spleen and lung were routinely made into thin sections for electron microscopic observation. (2) Spleens were harvested at 2, 8, 1, 6 and 24 h after intraperitoneal injection of 3 AU / kg of Acutobin in rats (N = 4) Pulmonary specimens were observed electron microscopy. Results Vioguain 1 AU / kg intravenous group of rabbits, platelets protruding a few pseudopods, individual agglutination or adhesion. However, internal structures such as dense bodies, alpha-granules, mitochondria, open tubules and dense tubules are still clearly visible. (3 AU / kg) dose group platelets protruding many processes, the internal structure disappeared in large numbers, only to see the dense body, suggesting that platelets are activated. At the same time, in rabbit and rat spleen, there are a large number of platelets with activated deactivation and disappearance of internal structure, suggesting platelet activation and being blocked in the spleen. Conclusion Acanthopanax enzyme intravenous injection in rabbits, the treatment of no effect on platelets, but the dose can be activated platelets, and finally degranulation was blocked in the spleen, resulting in reduced peripheral blood platelet count.