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本研究以高抗黄萎病的海岛棉品种海7124和高感黄萎病的陆地棉品种邯郸14组配的F2群体184个株系,构建了一个分子标记遗传连锁图,包括了142个位点和30个连锁群,标记间的平均距离为8.24cM,全长1169.6cM,覆盖棉花总基因组约23.4%。用复合区间作图分析共检测到12个抗黄萎病相关QTL,其中田间抗病性检测到不同时期7个病情指数相关QTL,1个病株率相关QTL,温室苗期抗病性定位4个病株率相关QTL。从绝对量上看,各QTL加性效应从2.20到42.39,显性效应从1.65到32.25,解释表型变异1.09%～22.73%,且田间抗病性获得的QTL与温室苗期抗病性得到的QTL基本相同,其中qFDI711-30-0.01位点距MUSS294仅为0.01cM,加性效应较高解释表型变异22.32%,这对于培育优质抗病材料用于标记辅助育种具有一定的参考价值。
In this study, we constructed 184 linkage groups of F2 population with 184 lines of F2 population, which were highly resistant to Verticillium wilt, sea island cotton variety 7121 and highly susceptible to Verticillium wilt disease. Point and 30 linkage groups, the average distance between markers was 8.24cM, total length of 1169.6cM, covering about 23.4% of the total cotton genome. A total of 12 QTLs for resistance to Verticillium wilt were detected by composite interval mapping analysis. Among them, 7 QTLs for disease index and 1 QTL for QTLs were detected in the field, A strain rate related QTL. In absolute terms, the additive effects of each QTL ranged from 2.20 to 42.39 and the dominant effects ranged from 1.65 to 32.25, accounting for 1.09% to 22.73% of the phenotypic variation, and the QTLs for disease resistance in the field and the disease resistance in greenhouse were obtained The QTLs for qFDI711-30-0.01 locus were only 0.01 cM away from MUSS294. The high additive effect explained 22.32% of the phenotypic variance, which is of certain value for breeding high-quality disease-resistant materials for marker-assisted breeding.