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目的采用HPLC法测定犬血浆中喹诺酮类先导化合物14a的浓度。方法以莫西沙星作为内标,血浆样品经二氯甲烷萃取后,采用HPLC法进行测定,色谱柱为Welchrom DOS-C18(200 mm×4.6 mm,5μm),流动相为乙腈-水(22∶78,磷酸调p H2.6),检测波长273 nm,流速1 m L·min-1。结果血浆中14a的线性范围为0.5~10.0μg·m L-1(r=0.9989),平均提取回收率为98.27%,日内RSD≤5.10%,日间RSD<15%。结论所用方法的专属性强、准确、灵敏、简便,适用于测定生物样品中14a的浓度。
Objective To determine the concentration of quinolone lead compound 14a in dog plasma by HPLC. Methods Moxifloxacin was used as an internal standard. The plasma samples were extracted with dichloromethane and determined by HPLC. The column was Welchrom DOS-C18 (200 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-water (22: 78, phosphorylation p H2.6), detection wavelength 273 nm, flow rate 1 m L · min-1. Results The linear range of plasma 14a was 0.5-10.0 μg · mL -1 (r = 0.9989). The average extraction recovery was 98.27%. The RSD was 5.10% and the intraday RSD was less than 15%. Conclusion The method used is specific, accurate, sensitive and simple, and is suitable for the determination of the concentration of 14a in biological samples.