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目的 一种预测化疗药物在肿瘤患者体内杀伤癌细胞功效的实验研究途径。方法 经采用形态学观察 ,MTT方法和流式细胞术分别检测 31例肿瘤患者经酞素D化疗后 1h ,4h及 1 2h的外周血血清中药物 (简称药物血清 )对体外培养的肺腺癌细胞系SPCA 1的抑癌作用。结果 经显微镜观察发现肿瘤患者化疗后不同时间的外周血中以 1h药物血清为最具有杀伤癌细胞的功效。流式细胞术检测结果与上述其他各种检测所获结果相符 ,1h药物血清组可导致 50 %～ 73 .5 %体外培养的SPCA 1细胞凋亡 ,而 4h及 1 2h组与对照组 (用药前血清 )均未见有明显杀伤癌细胞的作用。MTT法检测显示 31例患者中2 0例 (64 .5 % )的 1h药物血清对体外培养SPCA 1细胞具有明显杀伤肿瘤细胞的功效 ,而 4h组、1 2h组及对照组均为阴性结果。结论 鉴于肿瘤患者在化疗药物进入体内 1h后药物血清能对体外培养的肿瘤细胞呈现攻击作用 ,从而该检测方法可作为预测各种不同化疗药物对肿瘤患者在体内化疗疗效的一种客观的指标
OBJECTIVE: An experimental approach to predict the efficacy of chemotherapeutic drugs in killing tumor cells in cancer patients. Methods The morphological observation, MTT assay and flow cytometry were used to detect the effects of peripheral blood serum drugs (referred to as drug serum) at 1h, 4h and 12h after the treatment of phthalocyanine D on the proliferation of lung adenocarcinoma Inhibitory effect of cell line SPCA 1. Results The results of microscopic observation showed that 1h drug serum in the peripheral blood of tumor patients at different times after chemotherapy was the most effective for killing cancer cells. The result of flow cytometry was in good agreement with the results of other tests mentioned above. 1h drug serum group could induce the apoptosis of SPCA 1 cells in 50% -73.5% Pre-serum) showed no significant role in killing cancer cells. The results of MTT assay showed that 20% (64.5%) of 1h serum from 31 patients had obvious cytotoxic effect on SPCA 1 cells in vitro, while the 4h, 12h and control groups were negative results. Conclusion In view of tumor patients in the 1h after the entry of chemotherapeutic drugs serum can exert an attack on tumor cells cultured in vitro so that the detection method can be used as an objective indicator for predicting the efficacy of various chemotherapeutic drugs in chemotherapy in vivo