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[目的]研究黄芪多糖(APS)对肾阳虚型糖尿病大鼠肾组织核因子κB(NF-κB)mRNA和细胞内抑制性蛋白κB(IκB)mRNA的影响。[方法]将纯种SD雄性大鼠随机分为4组:对照组,模型组,苯那普利治疗组,APS治疗组。实验第8周时统一处死,以RT-PCR法检测各组大鼠肾组织NF-κB mRNA和IκB mRNA表达,并测定大鼠肾重/体重,血糖(BS)、血脂及肾功能。[结果]与苯那普利相比,APS能减少大鼠肾组织NF-κB mRNA表达,增加IκB mRNA表达,并可明显降低肾重/体重、BS、血脂,改善肾功能。[结论]APS能上调IκB mRNA表达,抑制NF-κB mRNA的过度表达,从而延缓肾阳虚型糖尿病肾病的进展。
[Objective] To study the effect of astragalus polysaccharides (APS) on renal nuclear factor-κB (NF-κB) mRNA and intracellular inhibitory protein κB (IκB) mRNA in kidney-deficiency diabetic rats. [Methods] Purebred SD male rats were randomly divided into 4 groups: control group, model group, benazepril treatment group and APS treatment group. At the 8th week of the experiment, they were sacrificed uniformly. RT-PCR was used to detect the expression of NF-κB mRNA and IκB mRNA in renal tissue of rats in each group. Rat kidney weight/body weight, blood glucose (BS), blood lipid and renal function were measured. [Results] Compared with benazepril, APS reduced the expression of NF-κB mRNA in kidney tissue of rats, increased the expression of IκB mRNA, and significantly decreased kidney weight/body weight, BS, blood lipid, and improved renal function. [Conclusion] APS can up-regulate the expression of IκB mRNA and inhibit the over-expression of NF-κB mRNA, thus delaying the progression of kidney-yang deficiency diabetic nephropathy.