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目的探讨妊娠期高血压疾病患者胎盘组织中血管活性肽 Apelin 的表达及其意义。方法选择妊娠期高血压疾病患者36例(妊娠期高血压疾病组),其中妊娠期高血压10例,轻度子痫前期11例,重度子痫前期15例;选择同期正常产妇15例作为正常妊娠组。采用免疫组化染色法及实时荧光定量 RT-PCR 分别检测两组产妇胎盘组织中 Apelin-36及 Apelin mRNA 的表达。结果正常妊娠组 Apelin-36及 Apelin mRNA 分别为0.27±0.04及0.82±0.25,妊娠期高血压疾病组分别为0.18±0.05及0.31±0.21,两组比较,差异有统计学意义(P<0.01);其中,妊娠期高血压患者分别为0.24±0.02及0.59±0.16,轻度子痫前期患者分别为0.16±0.03及0.25±0.07,重度子痫前期患者分别为0.14±0.02及0.17±0.09,轻度、重度子痫前期患者的 Apelin-36及 Apelin mRNA 表达水平均低于妊娠期高血压患者(P<0.01,P<0.01),轻度子痫前期患者与重度子痫前期患者比较,差异也有统计学意义(P<0.05,P<0.05)。结论血管活性肽 Apelin 在胎盘组织中表达降低,可能与妊娠期高血压疾病的发生、发展有关。
Objective To investigate the expression of vasoactive peptide Apelin in placenta of patients with gestational hypertension and its significance. Methods Thirty-six patients with gestational hypertension (gestational hypertension group) were included in the study. Among them, 10 cases of gestational hypertension, 11 cases of mild preeclampsia and 15 cases of severe preeclampsia; 15 cases of normal maternal pregnancy Pregnancy group. Immunohistochemistry and real-time fluorescent quantitative RT-PCR were used to detect the mRNA expression of Apelin-36 and Apelin in placenta of the two groups. Results The Apelin-36 and Apelin mRNA levels in normal pregnancy were 0.27 ± 0.04 and 0.82 ± 0.25 respectively, while those in gestational hypertension were 0.18 ± 0.05 and 0.31 ± 0.21, respectively (P <0.01) ; Among them, the patients with gestational hypertension were 0.24 ± 0.02 and 0.59 ± 0.16 respectively, the patients with mild preeclampsia were 0.16 ± 0.03 and 0.25 ± 0.07 respectively, and those with severe preeclampsia were 0.14 ± 0.02 and 0.17 ± 0.09 respectively The levels of Apelin-36 and Apelin mRNA in patients with severe preeclampsia and severe preeclampsia were lower than those in patients with gestational hypertension (P <0.01, P <0.01). There was also significant difference between patients with mild preeclampsia and severe preeclampsia Statistical significance (P <0.05, P <0.05). Conclusions The expression of vasoactive peptide Apelin in placenta decreased, which may be related to the occurrence and development of hypertensive disorders in pregnancy.