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以2年生猴樟幼苗为试验材料,采取人工模拟不同的降温幅度、降温用时、低温持续时间以及温度回升用时,测定每一阶段猴樟叶片的相对电导率、MDA含量、SOD活性、CAT活性、脯氨酸的含量。结果表明:降温幅度越大、低温持续时间越长,猴樟叶片MDA含量越高;相对电导率的测定结果表明猴樟生长胁迫最强的组合是A3B2C3D3,即降温幅度20℃+降温用时24 h+低温持续时间72 h+升温用时72 h;在温度下降后,SOD和CAT的活性均迅速增强,降幅为20℃时二者活性最强;20℃的降温处理,脯氨酸在含量增幅较大,且在同一降幅下,降温用时短的处理脯氨酸含量增幅较小。
The relative electric conductivity, MDA content, SOD activity and CAT activity in each stage of C. camphorata were measured by using two-year-old C. camphorata seedlings as test materials and simulating different temperature range, cooling time, low temperature duration and temperature recovery. Proline content. The results showed that: the larger the range of temperature decrease, the longer the duration of low temperature, the higher the content of MDA in C. camphora; the relative conductivity measured results showed that the strongest combination of growth of C. camphora was A3B2C3D3, ie, the temperature was 20 ℃ + 24 h + The duration of low temperature was 72 h and the time of heating was 72 h. After the temperature decreased, the activities of SOD and CAT increased rapidly, and the activity of SOD and CAT decreased sharply at 20 ℃. When the temperature was 20 ℃, the content of proline increased greatly, Under the same decline, the content of proline in short-time treatment with less temperature had a smaller increase.