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目的 :探讨运动后恢复过程中骨骼肌α 肌动蛋白基因的表达及运动对该基因表达的影响。方法 :设计强化训练的大鼠模型 ,用定量反转录聚合酶链式反应 (QuantitativeRT PCR)方法 ,研究恢复过程中 ,大鼠骨骼肌α 肌动蛋白 (α actin)基因的表达。结果 :所有强化训练运动恢复组的骨骼肌α 肌动蛋白mRNA水平均高于安静对照组 (C) ,且强化训练恢复安静组 (ER)、强化训练恢复 30min组 (E 0 .5 )显著高于安静对照组 (P <0 .0 5 )。强化训练运动后恢复即刻组 (E O)和强化训练运动后恢复 3h组 (E 3)较运动训练安静组 (ER)也有显著性提高 (P <0 .0 5 )。结论 :训练的确提高了大鼠骨骼肌α 肌动蛋白的mRNA水平 ,且运动恢复过程中骨骼肌α 肌动蛋白基因的表达具有时效性。
Objective: To investigate the expression of α-actin gene of skeletal muscle during exercise recovery and the effect of exercise on the gene expression. Methods: The rat model of intensive training was designed. Quantitative RT-PCR was used to study the expression of α-actin gene in skeletal muscle of rats. Results: The mRNA levels of α-actin in skeletal muscle of all intensive exercise training groups were significantly higher than that of the quiet control group (C), and were significantly higher in intensive training and recovery of quiet group (E0.5) In the quiet control group (P <0.05). There was also a significant increase (P <0. 05) in the immediate recovery group (E O) after intensive training exercise and the recovery of 3 h group (E 3) after intensive training exercise compared with the exercise-training quiet group (ER) CONCLUSIONS: Training did indeed increase α-actin mRNA levels in skeletal muscle of rats, and the skeletal α-actin gene expression was time-dependent during exercise recovery.