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目的探讨脑钠肽对高血脂大鼠缺血再灌注心肌的保护作用。方法高血脂SD大鼠30只,随机分为假手术组、缺血再灌注组和观察组各10只,缺血再灌注组和观察组大鼠制备心肌缺血再灌注模型,假手术组仅开胸不结扎冠状动脉;再灌注开始前5min观察组静脉注射0.01μg/(kg·min)脑钠肽,假手术组和缺血再灌注组静脉注射等量生理盐水。再灌注3h后观察3组大鼠心肌梗死面积和心肌细胞凋亡情况,检测3组大鼠血清肌酸激酶(creatine kinase,CK)、超氧化物歧化酶(superoxide dismutase,SOD)及丙二醛(malondialdehyde,MDA)水平。结果假手术组无明显心肌梗死区,缺血再灌注组心肌梗死面积[(38.45±2.20)%]和心肌细胞凋亡指数[(30.85±5.41)%]明显大于观察组[(24.16±3.51)%、(20.63±6.60)%]和假手术组[0、(2.20±0.61)%](P<0.05),观察组大于假手术组(P<0.05);缺血再灌注组和观察组大鼠血清CK[(810.06±37.64)、(743.21±41.73)u/L]、SOD[(53.05±11.59)、(70.13±13.20)ku/L]和MDA[(7.61±1.21)、(5.36±1.37)mol/L]水平均明显高于假手术组[(220.16±21.50)u/L、(20.51±6.71)ku/L、(0.91±0.13)mol/L](P<0.05);观察组大鼠血清CK和MDA水平明显低于缺血再灌注组(P<0.05),SOD水平明显高于缺血再灌注组(P<0.05)。结论脑钠肽对高血脂大鼠缺血再灌注心肌有保护作用,可减少心肌细胞凋亡,缩小心肌梗死面积,降低大鼠血清CK、MDA水平,增高血清SOD水平。
Objective To investigate the protective effect of brain natriuretic peptide on myocardial ischemia-reperfusion in hyperlipidemic rats. Methods Thirty SD rats with hyperlipidemia were randomly divided into sham-operated group, ischemia-reperfusion group and observation group, with 10 rats in each group. Rats in ischemia-reperfusion group and observation group were prepared for myocardial ischemia-reperfusion. The rats in the thoracotomy group were not ligated with coronary artery. The rats in the observation group were injected with 0.01μg / (kg · min) brain natriuretic peptide 5 minutes before reperfusion, and the rats in the sham operation group and ischemia-reperfusion group were given the same amount of normal saline. After 3 hours of reperfusion, myocardial infarct size and myocardial cell apoptosis were observed in 3 groups. Serum creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (malondialdehyde, MDA) levels. Results There was no significant myocardial infarction area in the sham operation group. The area of myocardial infarction [(38.45 ± 2.20)%] and the apoptosis index (30.85 ± 5.41)% in the ischemia / reperfusion group were significantly higher than those in the observation group [(24.16 ± 3.51) (20.63 ± 6.60)%] and sham group [0, (2.20 ± 0.61)%] (P <0.05), and the observation group was larger than the sham operation group (P <0.05). The ischemia / reperfusion group and the observation group The serum CK [(810.06 ± 37.64), (743.21 ± 41.73) u / L], SOD [(53.05 ± 11.59), (70.13 ± 13.20) ku / L] and MDA [(7.61 ± 1.21), (5.36 ± 1.37 ) were significantly higher than those in the sham operation group [(220.16 ± 21.50) u / L, (20.51 ± 6.71) ku / L, (0.91 ± 0.13) mol / L] The levels of serum CK and MDA in serum were significantly lower than those in ischemia-reperfusion group (P <0.05). The level of SOD in serum was significantly higher than that in ischemia-reperfusion group (P <0.05). Conclusions Brain natriuretic peptide can protect myocardium from ischemia-reperfusion injury in hyperlipidemic rats, reduce myocardial cell apoptosis, reduce myocardial infarct size, decrease serum CK and MDA, and increase serum SOD.