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目的探讨skp2、PTEN和p27在胶质瘤中的表达及其意义,及其相互关系。方法用免疫组织化学方法检测33例胶质瘤和6例正常脑组织标本中Skp2、PTEN和p27蛋白的表达。结果Skp2在正常脑组织中表达不明显,在低级别胶质瘤中表达较少,在高级别胶质瘤中表达较多,两两比较差别有统计学意义(P＜0．01)。PTEN和p27在正常脑组织中表达明显,在低级别胶质瘤中表达较少,在高级别胶质瘤中表达不明显,两两比较差异有统计学意义(P＜0．01)。胶质瘤中skp2蛋白表达与p27蛋白表达呈负相关(r=-0．809,P＜0．01),PTEN蛋白和p27蛋白的表达呈正相关(r=0．865,P＜0．01)。Skp2蛋白和PTEN蛋白的表达呈负相关(r=-0．814,P＜0．01)。结论在胶质瘤中PTEN的缺失突变导致Skp2在胶质瘤中表达升高,进一步促进p27的泛素化降解,使p27表达降低,失去对细胞周期的调控,从而促进胶质瘤的恶性进展和增殖。
Objective To investigate the expression and significance of skp2, PTEN and p27 in gliomas and their relationship. Methods The expressions of Skp2, PTEN and p27 proteins in 33 gliomas and 6 normal brain tissues were detected by immunohistochemistry. Results Skp2 was not expressed in normal brain tissue, less expressed in low grade glioma, and more in high grade glioma (P <0.01). The expressions of PTEN and p27 in normal brain tissue were significantly lower than those in low grade glioma and no significant difference in high grade gliomas (P <0.01). The expression of skp2 protein in glioma was negatively correlated with the expression of p27 protein (r = -0.809, P <0.01). The expression of PTEN protein and p27 protein was positively correlated (r = 0.865, P <0.01) ). Skp2 protein and PTEN protein expression was negatively correlated (r = -0.814, P <0.01). Conclusions The deletion mutation of PTEN in glioma leads to the increased expression of Skp2 in glioma, further promotes the degradation of p27 ubiquitination, decreases the expression of p27, and loses the regulation of cell cycle so as to promote the malignant progression of glioma And proliferation.