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The dynamics of chlorophyll content, leaf area and photosynthesis of cucumber seedlings were studied under sole stress of two low temperatures and low light intensity as well as combined stresses of low light intensity and the two low temperatures. The results showed that low light intensity reduced sensitivity of cucumber to low temperature and improved chlorophyll content, leaf area and chlorophyll fluorescence quantum yield. The photosynthesis rate was reduced under low light intensity. The intensity of light played the leading role in growth of cucumber under the low temperature condition, while the low temperature played the leading role under the critical low temperature condition. There were differences in reaction to light and temperature among different varieties. The tolerance to low temperature and low light intensity was not always synergetic for the same cucumber variety.