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目的筛选卵巢上皮性癌(卵巢癌)转移相关蛋白,进一步探讨卵巢癌的转移机制。方法以卵巢癌细胞系HO-8910和高转移卵巢癌细胞系HO-8910PM为研究对象,采用比较蛋白组学的研究技术(双向电泳、质谱分析),筛选两个细胞系中差异表达的蛋白质。结果高转移细胞系HO-8910PM与卵巢癌细胞系HO-8910比较,共出现了21个差异表达蛋白质点,其中16个蛋白质点表达上调,5个蛋白质点表达下调。21个差异表达蛋白质点有17个被鉴定,并分为7大类,即锌指蛋白、钙结合蛋白、DNA修复及合成蛋白、细胞调节蛋白、细胞代谢相关蛋白、细胞信号和传导蛋白及细胞表面抗原。结论卵巢癌细胞系HO-8910和高转移卵巢癌细胞系HO-8910PM的蛋白质表达存在明显差异。
Objective To screen the metastasis - associated proteins of epithelial ovarian cancer (ovarian cancer) and further explore the metastatic mechanism of ovarian cancer. Methods The ovarian cancer cell line HO-8910 and the highly metastatic ovarian cancer cell line HO-8910PM were used as research objects, and comparative proteomics research techniques (two-dimensional electrophoresis and mass spectrometry) were used to screen the differentially expressed proteins in two cell lines. Results Compared with ovarian cancer cell line HO-8910, HO-8910PM showed a total of 21 differentially expressed protein spots, of which 16 were up-regulated and 5 were down-regulated. Twenty-one of 21 differentially expressed protein spots were identified and grouped into seven broad categories: zinc finger proteins, calcium-binding proteins, DNA repair and synthesis proteins, cell-regulatory proteins, cell-metabolism related proteins, cell signaling and conduction proteins, and cells Surface antigen. Conclusion The protein expression of ovarian cancer cell line HO-8910 and highly metastatic ovarian cancer cell line HO-8910PM is significantly different.