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目的：探索外源性细胞因子 TNF-α基因克服多药耐药（ multidrug resistance,MDR）的新途径。方法：以重组逆转录病毒为载体，将 TNF-α基因导入具 MDR表型的人乳腺癌细胞系 MCF- 7/Adr，经 G418抗性筛选获阳性克隆 MCF- 7/Adr- TNF1与 MCF- 7/Adr- TNF2。以 PCR和 ELISA法检测目的基因的整合与表达。细胞计数法观察细胞生长速度的改变 , MTT法检测外源性 TNF-α基因的逆转 MDR作用，流式细胞仪分析细胞内 ADR积累的变化。结果： MCF- 7/Adr- TNF1与 MCF- 7/Adr- TNF2细胞中有 TNF-α基因的整合和表达，病毒上清中 TNF-α含量分别为 1 737 pg/ml（ 106 cells/48 h）、 2 875 pg/ml。与阴性对照细胞相比， MCF- 7/Adr- TNF1与 MCF- 7/Adr- TNF2细胞的生长速度明显减慢，生长抑制率分别为 32.4％、 54.8％，对 ADR的耐药性明显降低，耐药逆转倍数分别为 5.2倍、 19.3倍，细胞内 ADR的积累明显增加。结论：外源性 TNF-α基因的导入能有效克服耐药性，增加细胞内药物的积累为其逆转耐药性的主要机制。
Objective: To explore a new way to overcome multidrug resistance (MDR) by exogenous cytokine TNF-α gene. Methods: Recombinant retrovirus was used to transduce TNF-α gene into human breast cancer cell line MCF-7 / Adr with MDR phenotype. The positive clones MCF-7 / Adr-TNF1 and MCF- 7 / Adr-TNF2. The integration and expression of the target gene were detected by PCR and ELISA. Cell counting was used to observe the changes of cell growth rate. MTT assay was used to detect the reverse MDR effect of exogenous TNF-α gene. The changes of intracellular ADR accumulation were analyzed by flow cytometry. Results: The integration and expression of TNF-α gene in MCF-7 / Adr-TNF1 and MCF-7 / Adr-TNF2 cells showed that the concentration of TNF-α in virus supernatant was 1 737 pg / ml ), 2 875 pg / ml. Compared with the negative control cells, the growth rate of MCF-7 / Adr-TNF1 and MCF-7 / Adr-TNF2 cells were significantly slowed down and the growth inhibition rates were 32.4% and 54.8%, respectively. The reversal times of resistance were 5.2 times and 19.3 times respectively, and the accumulation of intracellular ADR increased obviously. Conclusion: The introduction of exogenous TNF-α gene can effectively overcome drug resistance and increase the accumulation of intracellular drugs as the main mechanism of reversing drug resistance.