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[目的]探讨锰接触工人神经行为改变与Parkin基因7号外显子Gly284Arg多态性的相关性。[方法]选工龄在半年以上的职业性锰暴露工人200名和劳动强度相似,但工作环境不接触锰工人94名,按猛累积暴露指数将其分为高、低猛暴露组和对照组。利用沟槽稳定试验和拼板试验进行神经行为改变检测;利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对Parkin基因7号外显子多态性位点进行基因分型。[结果]高暴露组中,Parkin基因7号外显子CC型工人的划痕长度、拼板试验得分均低于GG型和GC型,而碰撞次数高于GG型和GC型,其差别有统计学意义(P<0.05);低暴露组中,Parkin基因7号外显子CC型工人的划痕长度、拼板试验得分均低于GG型和GC型,其差别有统计学意义(P<0.05),3个基因型工人碰撞次数间差别无统计学意义(P>0.05);对照组中,Parkin基因7号外显子3个基因型划痕长度、碰撞次数、拼板试验得分之间差异无统计学意义(P>0.05)。[结论]环境因素和遗传因素共同作用更易导致锰引起神经行为改变;携带Parkin基因7号外显子CC基因型的工人是锰损伤的易感者;Parkin基因7号外显子G951C突变导致Gly284Arg多态性有可能作为检测锰致神经行为改变以及锰中毒风险评估的指标。
[Objective] To investigate the correlation between neurobehavioral changes in patients exposed to manganese and the Gly284Arg polymorphism of exon 7 of Parkin gene. [Methods] 200 occupational manganese exposure workers whose working age was over six months were similar to labor intensity, but 94 workers were not exposed to the working environment. They were divided into high and low exposure groups and control groups according to their cumulative exposure index. The neurobehavioral changes were detected by groove stabilization test and panel test. The polymorphism of exon 7 of Parkin gene was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . [Results] The scratch length and the panel test scores of Parkin gene exon 7 CC genotype were lower than that of GG genotype and GC genotype in high exposure group, but the number of collisions was higher than that of GG genotype and GC genotype (P <0.05). In the low exposure group, the scratch length and the panel test scores of Parkin gene exon 7 CC genotype were lower than those of GG and GC genotypes (P <0.05) ), And there was no significant difference in the number of collisions between the three genotypes (P> 0.05). In the control group, there was no significant difference between the three genotypes of Parkin gene in the length of the three genotypes, the number of collisions and the number of panel test scores Statistical significance (P> 0.05). [Conclusion] The combination of environmental factors and genetic factors may lead to the change of neurobehavioral behavior of manganese. The workers carrying the genotype CC of exon 7 of Parkin gene are susceptible to manganese injury. The mutation of G951C of exon 7 of Parkin gene results in the polymorphism of Gly284Arg Sex may be used as an indicator of behavioral changes in manganese induced neurotoxicity and assessment of the risk of manganese poisoning.