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目的制作脱氧雪腐镰刀菌烯醇(DON)胎鼠模型,观察DON对小鼠的致骨骼畸形作用和胚胎毒性,为进一步研究DON致畸的机理提供试验平台。方法将妊娠小鼠分为溶剂对照组和DON实验组,每组6只孕鼠。实验组小鼠于孕期第7~10 d连续腹腔注射DON,每天2.5 mg/kg体重,总剂量为10 mg/kg体重。溶剂对照组注射30%丙二醇。妊娠第18 d解剖孕鼠,实验组随机取31只胎鼠,溶剂对照组取63只胎鼠,去除软组织,进行骨骼、软骨双重染色,镜下观察骨骼发育情况。结果实验组31只胎鼠均出现多发性骨骼畸形,其中胸骨畸形率为90.32%,胸椎畸形率90.32%,四肢畸形率为54.84%。溶剂对照组胎鼠胸骨畸形率为9.52%,未见其他骨畸形。结论DON可致胎鼠多发性骨骼畸形;DON小鼠模型可用于骨骼先天性畸形研究。
Objective To prepare deoxynivalenol (DON) fetus mouse model to observe the effect of DON on skeletal malformation and embryotoxicity in mice, and to provide experimental platform for further study on the mechanism of DON teratogenicity. Methods Pregnant mice were divided into solvent control group and DON experimental group, 6 pregnant rats in each group. The mice in experiment group were injected intraperitoneally with DON every day for 7-10 d, 2.5 mg / kg body weight per day for a total dose of 10 mg / kg body weight. The solvent control group was injected with 30% propylene glycol. Pregnant rats were dissected on the 18th day of gestation. Totally 31 fetuses were randomly selected from the experimental group. Totally 63 fetuses were removed from the control group. The soft tissues were removed and the bone and cartilage were double stained. The bone development was observed microscopically. Results In the experimental group, all 31 fetuses had multiple skeletal deformities. The rate of sternal deformity was 90.32%, the rate of thoracic deformity was 90.32% and the rate of limb deformity was 54.84%. Solvent control group of fetal rat sternal deformity rate was 9.52%, no other bone deformities. Conclusion DON can cause multiple skeletal deformities in the fetus. The DON mouse model can be used for the study of congenital malformation of the skeleton.