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目的：了解ｐ１６基因在急性髓系白血病（ＡＭＬ）发病中的意义。方法：用聚合酶链反应－单链ＤＮＡ构象多态性、Ｎｏｒｔｈｅｒｎｂｌｏｔ方法分析１６例ＡＭＬ细胞ｐ１６基因结构及转录水平表达。结果：１６例均无ｐ１６基因的缺失、突变。初治和复发的１２例ＡＭＬ的ｐ１６ｍＲＮＡ表达水平明显低于４例长期缓解者及正常对照。１例ｐ５３基因突变ＡＭＬ的ｐ１６ｍＲＮＡ有明显升高。结论：ｐ１６基因在ＡＭＬ发病中可能不占重要地位，而在ＡＭＬ病态造血的维持中起一定作用，在ｐ５３突变时其表达反馈性升高，显示一种反馈环路失衡，提示在研究单个基因的基础上观察细胞周期检测点功能具有重要意义。
Objective: To understand the significance of p16 gene in the pathogenesis of acute myeloid leukemia (AML). Methods: The p16 gene structure and transcriptional expression of 16 AML cells were analyzed by polymerase chain reaction-single strand DNA conformation polymorphism and Northern blot analysis. Results: There was no deletion or mutation of p16 gene in 16 cases. The expression of p16 mRNA in 12 newly diagnosed and relapsed AML patients was significantly lower than that of 4 long-term remission and normal controls. One p53 gene mutation in AML showed a significant increase in p16 mRNA. Conclusion: p16 gene may not play an important role in the pathogenesis of AML, but play a role in the maintenance of anaplastic hematopoiesis in AML. Its expression feedback is increased when p53 is mutated, indicating a feedback loop imbalance, suggesting that a single gene is being studied. Based on the observation of cell cycle detection point function is of great significance.