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为探索飞秒激光在兔眼巩膜上产生光离解作用的可行性,并寻找适当的激光切割方式及相关参数,将不同脉冲能量的飞秒激光(800nm/50fs)聚焦后作用于离体兔眼巩膜。通过计算机控制的三维平台的定向移动,飞秒激光能够在兔眼巩膜上完成打孔、蛇形扫描和线性切割三种方式的光离解作用。应用光学显微镜和扫描电镜(SEM)观察激光作用后巩膜的形态学变化,并用Nd∶YAG激光作为对比。实验结果表明,飞秒激光经过显微物镜(NA0.2)聚焦后,当其功率密度达到或超过9.55×1014W/cm2,脉冲能量在37.5~125μJ变化时,激光以0.1mm/s的速度线性扫描巩膜能形成深度为30~70μm的沟道;当激光的功率密度减小至7.96×1014W/cm2,脉冲能量小于31.25μJ/pulse时,在相同条件下却不能产生光离解作用。与Nd∶YAG激光相比,飞秒激光在兔眼巩膜上切割瘘道的内壁更加光滑整齐,对周围的组织损伤更小。飞秒激光对离体兔眼巩膜高精度和微创伤的光离解作用,预示了它在未来青光眼治疗中有潜在的应用价值。
In order to explore the feasibility of femtosecond laser photodissociation in sclera of rabbits and to find the appropriate laser cutting mode and related parameters, different impulse energy femtosecond laser (800nm / 50fs) was focused on isolated rabbit eyes sclera. Through the computer-controlled directional movement of the three-dimensional platform, the femtosecond laser can complete the photodissociation of three ways of drilling, serpentine scanning and linear cutting on the sclera of the rabbit eye. The morphological changes of the sclera after laser irradiation were observed by optical microscope and scanning electron microscope (SEM), and Nd: YAG laser was used as the contrast. The experimental results show that when the power of the femtosecond laser is focused at NA0.2, the power of the femtosecond laser reaches 9.55 × 1014 W / cm2 or more and the pulse energy changes from 37.5 to 125 μJ, the laser is linearly linearized at 0.1 mm / s Scanning the sclera can form a channel with a depth of 30-70μm. When the laser power density is reduced to 7.96 × 1014W / cm2 and pulse energy is less than 31.25μJ / pulse, it can not produce photodissociation under the same conditions. Compared with the Nd: YAG laser, femtosecond laser cutting the sclera in the sclera of the rabbit’s eye is smoother and more tidy, with less damage to the surrounding tissue. The photodissociation of femtosecond laser on the high-precision and minimally invasive trabecular sclera in vitro indicates its potential application in the future treatment of glaucoma.