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目的 考察本所精制抗江浙蝮蛇毒血清能否中和三种韩国蝮蛇毒及其中和能力。方法 用免疫双扩散法鉴定抗江浙蝮蛇毒血清与三种韩国蝮蛇毒交叉反应。并根据动物实验结果计算出每毫升抗血清中和三种韩国蝮蛇毒的致死毒及出血毒的量。结果 中国精制抗江浙蝮蛇毒血清 1.0ml可分别中和韩国黑眉蝮蛇毒、短尾蛇毒和乌苏里蝮蛇毒液态原毒的致死毒 1、3.9和 2 .5mg ;同时可分别中和上述 3种蛇毒蛋白 0 .8、3.15和 2 0mg中和出血毒。结论 中国精制抗江浙蝮蛇毒血清具有中和上述三种韩国蝮科蛇毒的作用。
Objective To investigate whether the purified anti-Jiang Zhe Zhe snake venom serum can neutralize three kinds of South Korean snake venom and its neutralization ability. Methods The double immunodiffusion method was used to identify the cross-reaction of anti-Jiang Zhe Zhe snake venom serum with three kinds of Korean viper venom. Based on the results of the animal experiments, the lethal and bleeding toxicity of three kinds of Korean viper venom were calculated per mL of antiserum. Results The results showed that the Chinese refined anti-Jiang Zhe Zhe snake venom serum 1.0ml neutralize the lethal toxicity of liquid poison of the original black-browed snake venom, the black-footed snake venom and the Wusuli viper venom respectively 1,3,9 and 2,5mg; at the same time, Snake venom protein 0.8, 3.15 and 20 mg neutralize bleeding poison. Conclusion The Chinese refined anti-Jiang Zhe Zhe snake venom serum has the role of neutralizing the above-mentioned three kinds of snake venom in Korea.