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目的:分析半夏高温倒苗过程中总RNA及mRNA差异表达的变化规律,为研究半夏高温倒苗的分子机制提供了信息。方法:经过不同时间的高温胁迫处理,测定半夏叶片总RNA含量变化和mRNA表达的差异。结果:半夏叶片总RNA的含量变化分为3个降低阶段和2个升高阶段,胁迫0,6 h时含量最高,胁迫4,42 h和倒苗时含量最低,差异显示表明胁迫6h的样品条带多态性和带型与0 h的接近,其余胁迫时间的样品条带远少于0,6 h时的样品条带,并且在不同的样品之间均有mRNA差异条带出现。结论:在半夏经高温胁迫而倒苗的过程中,基因表达开启与关闭和基因表达增强与减弱模式具有一定的规律,并且可能启动了不同的基因表达系统。
OBJECTIVE: To analyze the variation of total RNA and mRNA differential expression in Pinellia ternate under high temperature and to provide information for studying the molecular mechanism of Pinellia ternate underpowered. Methods: After high temperature stress treatment for different time, the content of total RNA in Pinellia ternata leaves and the difference of mRNA expression were determined. Results: The content of total RNA in Pinellia ternata leaves was divided into three stages of decreasing and two stages of increasing. The content of total RNA was the highest at 0 and 6 h, the lowest at 4,42 h and the time of sponging, the difference showed that under 6 h stress The polymorphism and banding pattern of the samples were similar to that of 0 h. The bands of the remaining stress time were far less than those of the samples at 0 and 6 h, and mRNA differential bands appeared between different samples. CONCLUSION: During the process of inversus through the process of high temperature stress, the gene expression is turned on and off, the gene expression is enhanced and weakened, and some gene expression systems may be initiated.