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目的研究正常人膀胱中细胞突触及其构成蛋白连接蛋白(Cx)是否存在。方法对6例全膀胱切除患者平滑肌用透射电子显微镜,冷冻爆裂电镜,逆转录聚合酶链反应(RT-PCR),免疫组化等方法进行研究。结果电镜结果显示膀胱平滑肌存在细胞突触,但这些细胞突触较小、形态不规律。RT-PCR 结果显示所有的样本均扩增出2个片段,大小分别为300 bp 和200 bp,测序结果显示它与已发表的 Cx45一致。荧光免疫组化显示大多数逼尿肌存在 Cx45的免疫信号。结论非梗阻性人膀胱平滑肌细胞是通过细胞突触进行电耦合的。
Objective To study the synapses of normal human bladder and the existence of connexin (Cx). Methods Six cases of total cystectomy patients with smooth muscle by transmission electron microscopy, cryo - bursting electron microscopy, reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry and other methods were studied. Results Electron microscopy showed that synapses existed in bladder smooth muscle cells, but the synapses of these cells were smaller and irregular in morphology. RT-PCR results showed that all the samples amplified two fragments of 300 bp and 200 bp, respectively, and the sequencing results showed that it was consistent with the published Cx45. Fluorescent immunohistochemistry showed that most detrusors had Cx45 immune signals. Conclusion Non-obstructive human bladder smooth muscle cells are electrically coupled via synaptic cells.