在研究戊二醛复方对乙型肝炎表面抗原(HBsAg)灭活作用时,观察了几种中和剂的效果。HBsAg的测定使用固相放射免疫法(SPRIA)。HBsAg(1mg/ml),为北京医科大学肝病研究所产,放免测定盒由北京生物制品研究所提供。试验时,将HBsAg用含5％小牛血清的磷酸盐缓冲液(pH7.2,0.01 M)稀释成10μg/ml。戊二醛复方中含戊二醛0.5％(w/v)。试验分2部份,一部份取0.4 ml中和剂加0.1 mlHBsAg,另一部份取4 ml中和剂加1 ml药 In the study of glutaraldehyde compound on hepatitis B surface antigen (HBsAg) inactivation, observed the effect of several neutralizers. The HBsAg assay uses solid-phase radioimmunoassay (SPRIA). HBsAg (1mg / ml), produced by the Institute of Hepatology, Beijing Medical University, radioimmunoassay kit provided by the Beijing Institute of Biological Products. For the test, HBsAg was diluted to 10 μg / ml with phosphate buffered saline (pH 7.2, 0.01 M) containing 5% fetal bovine serum. Glutaraldehyde compound containing glutaraldehyde 0.5% (w / v). Test points 2 parts, part of 0.4 ml neutralizer plus 0.1 ml HBsAg, the other part to take 4 ml neutralizer plus 1 ml of medicine