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NAC(NAM-ATAF1,2-CUC2)转录因子在植物胁迫响应中起重要作用。为了探讨LeNLP4基因在番茄抗低温胁迫中的功能,分离了番茄LeNLP4转录因子基因,并获得转正义LeNLP4基因番茄植株。荧光定量PCR分析表明,LeNLP4的表达受低温诱导。与野生型植株相比,在4℃胁迫下转基因植株具有较高的生长量和光系统Ⅱ(PSⅡ)最大光化学效率(F_v/F_m)、过氧化氢(H_2O_2)和超氧阴离子(O_2)清除速率、抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性,以及较低的丙二醛(MDA)含量和相对电导率(REC)。过表达株系中SlCBF1的表达高于野生型。上述结果表明,LeNLP4的过表达提高了转基因番茄抗低温胁迫能力。
NAC (NAM-ATAF1,2-CUC2) transcription factors play an important role in plant stress response. In order to investigate the function of LeNLP4 in tomato under low-temperature stress, the LeNLP4 transcription factor gene was isolated from tomato and the tomato plants with sense LeNLP4 gene were obtained. Fluorescent quantitative PCR analysis showed that the expression of LeNLP4 was induced by hypothermia. Compared with wild type plants, the transgenic plants had higher growth rate and maximum photochemical efficiency (F_v / F_m), hydrogen peroxide (H_2O_2) and superoxide anion (O_2) removal rate under PSⅡ stress at 4 ℃. , Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activity, as well as lower malondialdehyde (MDA) content and relative electrical conductivity (REC). The expression of SlCBF1 in overexpression lines was higher than that in wild type. The above results show that overexpression of LeNLP4 enhances the ability of transgenic tomato to resist cold stress.