论文部分内容阅读
用６０Ｃｏγ射线辐射处理小孢子处于单核靠边期的烟草Ｋ３２６、ＮＣ８９、８６１１花蕾并进行花药培养，获得的单倍体植株移栽成活后接种黄瓜花叶病毒病（ＣＭＶ）汁液。结果表明，ＮＣ８９、８６１１单倍体植株的抗病性变异与６０Ｃｏγ射线辐射剂量之间存在一定范围的定向性，剂量≥４ｋｒａｄ时，植株叶片变异与ＣＭＶ症状相似，不利于选择；剂量＜２ｋｒａｄ时，植株的抗病性与对照之间没有差异；剂量＝２ｋｒａｄ时，植株的抗病性与对照之间的差异可达到显著水平，并在２ｋｒａｄ处理的ＮＣ８９、８６１１单倍体植株群体中筛选到了抗ＣＭＶ突变体。
The flower buds of tobacco K326, NC89 and 8611 with single microsatellite edge were treated with 60Coγ ray and cultured in anther culture. The haploid plants were inoculated with cucumber mosaic virus (CMV) juice after transplanting. The results showed that there was a certain degree of orientation between the disease resistance variation of NC89 and 86611 haploid plants and the dose of 60Coγ-ray radiation. When the dose was> 4krad, the variation of plant leaves was similar to that of CMV, , There was no difference between the disease resistance of the plants and the control; the difference between the disease resistance of the plants and the control reached a significant level at the dose of 2krad and was screened in the 2krad-treated NC89,8611 haploid plant population Anti-CMV mutant.