论文部分内容阅读
目的:研究观察姜黄素联用VCR(长春新碱)对人结肠癌细胞凋亡的影响。方法:用培养基培养人结肠癌细胞(HT-29),将VCR单独作用于结肠癌细胞作为对照组,姜黄素与VCR共同作用为实验组,利用MTT比色法观察两组细胞的增殖情况,荧光染料双染法检测两组HT-29细胞的凋亡情况,并进行比较。结果:实验组HT-29细胞的增殖明显受到抑制,细胞的凋亡率明显比对照组高,存在统计学意义(P<0.05)。结论姜黄素与VCR共同作用能加强人结肠癌细胞的凋亡,可用于肿瘤的治疗。
Objective: To observe the effect of curcumin combined with VCR (vincristine) on human colon cancer cell apoptosis. Methods: Human colon cancer cells (HT-29) were cultured with culture medium, VCR alone was applied to colon cancer cells as control group, curcumin and VCR were used as experimental group, MTT colorimetry was used to observe the proliferation of the two groups of cells The apoptosis of HT-29 cells in two groups was detected by fluorochrome double staining and compared. Results: The proliferation of HT-29 cells in experimental group was significantly inhibited, and the apoptosis rate of HT-29 cells in experimental group was significantly higher than that in control group (P <0.05). Conclusion The combination of curcumin and VCR can enhance the apoptosis of human colon cancer cells and can be used in the treatment of tumors.