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研究了二苯乙烯荧光蓝－Ｓ荧光猝灭测定微量锌（Ⅱ）的实验条件．在ｐＨ６．０的Ｈ３ＰＯ４－Ｈ３ＢＯ３－ＨＡｃ－ＮａＯＨ缓冲溶液中，试剂的最大激发波长λｅｘ＝３６７ｎｍ，最大荧光发射波长λｅｍ＝４５７ｎｍ；Ｚｎ（Ⅱ）与试剂生成的１∶２的配合物使试剂的荧光强度减弱；利用荧光猝灭法测定微量锌（Ⅱ），线性范围为０～０．８μｇ／１０ｍｌ；方法用于测定食品中、人发中的微量锌（Ⅱ），结果满意．
The experimental conditions for the determination of trace zinc (Ⅱ) with stilbene blue-S fluorescence quenching were studied. In pH 6.0 H3PO4-H3BO3-HAc-NaOH buffer solution, the maximum excitation wavelength λex = 367nm, the maximum fluorescence emission wavelength λem = 457nm; the complex of Zn (Ⅱ) The fluorescence intensity was weakened. The trace zinc (Ⅱ) was detected by fluorescence quenching with a linear range of 0 ~ 0.8μg / 10ml. The method was applied to the determination of trace zinc (Ⅱ) in food and human hair with satisfactory results.