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目的 探讨外源性前列腺素E1(PGE1)对血吸虫病家兔肝脏血小板衍生生长因子B(PDGF B)及其受体表达的影响。方法 14只血吸虫病纤维化家兔模型,其中7只家兔在感染后6 0d开始给予PGE1( 2 5 μg/kg·d-1)至12 0d。RT PCR、Westernblot和免疫组化分别检测肝组织PDGF BmRNA、PDGFR β蛋白和α平滑肌肌动蛋白(αSMA)表达。原位杂交观察内源性干扰素(IFN)γ水平。结果 纤维化形成过程中HSCs活化,PDGF BmRNA以及PDGFR β蛋白水平增高。外源性PGE1治疗后PDGFmRNA和受体β蛋白表达的增高趋势都受到明显抑制(与模型组比较分别为2 9 4 2±5 0 5和4 1 37±7 2 3,P <0 0 1;0 2 1±0 11和0 4 6±0 0 7,P <0 0 1)。αSMA表达强阳性视野从6 8下降到10个;而内源性IFN γ积分光密度从0 10 5±0 0 2 4升高到0 4 38±0 0 76 (P <0 0 1)。结论 PGE1可以降低血吸虫病家兔肝脏PDGF B基因及其受体表达水平,内源性IFN γ水平上调可能是这种作用的机制之一。
Objective To investigate the effect of exogenous prostaglandin E1 (PGE1) on the expression of hepatic platelet-derived growth factor B (PDGF B) and its receptor in schistosomiasis rabbits. Methods Fourteen schistosomiasis rabbits were randomly divided into four groups. Seven rabbits were given PGE1 (25 μg / kg · d-1) to 120 days after the onset of infection. The expression of PDGF B mRNA, PDGFR beta protein and alpha smooth muscle actin (α SMA) in liver tissue were detected by RT PCR, Western blot and immunohistochemistry respectively. In situ hybridization was used to observe the level of endogenous interferon (IFN) γ. Results HSCs activation, PDGF B mRNA and PDGFR β protein increased during fibrosis. The increased tendency of PDGFmRNA and receptorβprotein expression was significantly inhibited after exogenous PGE1 treatment (compared with the model group, they were 2942 ± 5 05 and 4137 ± 723 respectively, P <0.01; 0 2 1 ± 0 11 and 0 4 6 ± 0 0 7, P <0 0 1). The positive field of αSMA expression decreased from 68 to 10, while the endogenous IFNγ integral optical density increased from 0 105 ± 0 024 to 0 388 076 (P 0 01). Conclusion PGE1 can reduce the expression of PDGF B gene and its receptor in the liver of schistosomiasis rabbits. Upregulation of endogenous IFN-γ may be one of the mechanisms of this effect.